Sailgene Technology
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Sailgene Technology
Introduction
SMAC-seq (Single-Molecule long-read Accessible Chromatin mapping sequencing assay) is a single-molecule resolution method for profiling chromatin accessibility across long genomic regions. This approach utilizes DNA methyltransferases that preferentially methylate accessible chromatin regions. By detecting these methylation marks through Oxford Nanopore sequencing platform, SMAC-seq enables direct identification of open chromatin regions and compacted domains. The technique provides high-resolution accessibility maps across kilobase-scale regions, allowing for quantitative assessment of chromatin states and spatial correlations between distal regulatory elements.
Applications
Genome-wide mapping of chromatin accessibility and co-accessibility
High-resolution nucleosome positioning
Transcription factor footprinting analysis
Dynamics of chromatin accessibility in dynamic processes
Highlights
Reveal connections between distal regulatory elements
High resolution at the single-molecule level
Identify open chromatin regions and nucleosome positions at the same time
Integrate other epigenomics data
Library Construction Workflow
Analysis Workflow
Data Delivery File Formats
Deliver data in POD5 format or FASTQ format.
Data Delivery Standard
Sample Requirements
|
Sample Type Platform |
Plant tissue sample |
Animal tissue sample |
Number of cells cultured |
Blood |
Bacterial sample |
fungi |
|
SMAC-seq |
≥500 mg |
≥500 mg |
≥2*106 |
≥5 mL |
- |
≥2000 mg |
Publications
|
Year |
Journal |
Impact Factor |
Title |
|
2024 |
Nucleic Acids Research |
13.1 |
Simultaneous profiling of chromatin accessibility and DNA methylation in complete plant genomes using long-read sequencing |
|
2023 |
nature plants |
13.6 |
Single-molecule targeted accessibility and methylation sequencing of centromeres,telomeres and rDNAs in Arabidopsis |
|
2022 |
Methods in Molecular Biology |
- |
Single-molecule multikilobase-scale profiling of chromatin accessibility using m6A-SMAC-seq and m6A-CpG-GpC-SMAC-seq |
|
2022 |
Nature Genetics |
29.0 |
Long-range phasing of dynamic, tissue-specific and allele_x0002_specific regulatory elements |
|
2020 |
Nature Methods |
32.1 |
Simultaneous profiling of chromatin accessibility and methylation on human cell lines with nanopore sequencing |
|
2020 |
Nature Methods |
32.1 |
Long-range single-molecule mapping of chromatin accessibility in eukaryotes |
What's the best choice?
| SMAC-seq | NOMe-seq | ATAC-seq Learn more>>> |
DNaseq | |
| Chromatin accessibility | Labeling with exogenous methyltransferases | Labeling with exogenous methyltransferases | Sequencing of enzymatic cleavage fragments | Sequencing of enzymatic cleavage fragments |
| nucleosome positioning | Direct detection | Direct detection | Indirect inference | Indirect inference |
| DNA methylation calling | 5mC、5hmc、6mA、4mc | 5mC | Not available | Not available |
| Single-molecule | Yes | No | No | No |
| Read Length | Long | Short | Short | Short |
Technical Comparison: SMAC-seq vs. Fiber-seq
|
Technical Points |
SMAC-seq |
Fiber-seq |
|
Reads N50 (post-run, successful libraries) |
≥15kb (guaranteed) |
~15kb (Fixed-length library) |
|
Per-flowcell Yield (post-run, successful libraries) |
Slightly lower than standard ONT whole-genome libraries; up to 80G guaranteed (sample-dependent) |
Slightly lower than standard HIFI whole-genome libraries; ~100G guaranteed for qualified routine samples |
|
Compatible Sample Types |
Routine samples and challenging species (no yield guarantee for challenging species) |
Routine samples only |
|
Ultra-long Read Mode |
Theoretically supported |
Not supported |
|
Targeted Sequencing Mode |
Theoretically supported |
Not supported |
|
DNA Methylation Analysis |
Three types (5mC, 5hmC, 4mA) |
Single type only (5mC) |
|
Coverage of High-Repetitive Regions (Centromeres, Telomeres) |
Supported, with validated literature and dedicated tool (MAGNIFIER) |
Not supported |
Contact Us
If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.
Contact Us
Add: 82 Wendell Avenue, Pittsfield, Massachusetts, USA
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