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    Classification:

    Single-Cell Transcriptome Sequencing


    Introduction


    10X Genomics single-cell RNA sequencing is a high-throughput cell capture technology based on microfluidics, droplet encapsulation, and barcode labeling. It enables the simultaneous isolation and labeling of 500–10,000 single cells, capturing the 3' or 5' transcriptome information of each cell. This approach allows for the study of gene expression profiles at high throughput and single-cell resolution, revealing the heterogeneity of complex cell populations and avoiding the masking of individual cell gene expression signals by population averaging.

    Applications


    Organs/Tissue Cell Atlas

    Developmental Biology Research

    Disease-Related Cell Type Discovery

    Cancer Research

    Virology Research

    Highlights


    Rare Cell Subtype Identification

    True Single-cell Resolution

    High Throughput

    Multi-Omics Integration

    Precision Medicine Potential

    Workflow


    Sample Preparation

    Sample Preparation

    Refer to the Sample
    Submission Requirements

    Sample QC

    Sample Preparation
    Single Nuclei Suspension Generation

    Single Nuclei Suspension Generation

    Refer to Single Nuclei
    Suspension Generation Demo Protocols

    Suspension QC

    Single Nuclei Suspension Generation
    Single Cell Library Preparation

    Single Cell Library Preparation

    · GEM (Gel Beads-In-Emulsion) generation and barcoding
    · cDNA synthesis & amplification
    · Short-read library construction

    Library QC

    Single Cell Library Preparation
    Sequencing

    Sequencing

    · Platform: Illumina or BGI
    · Strategy: PE150 or PE100
    · Data volume: 100 G

    Data QC

    Sequencing
    Bioinformatics Analysis

    Bioinformatics Analysis

    Refer to the demo report

    Click >>

    Analysis Workflow


    a7452dc4caa2a52dae7ad73881842b85_31f8e20a-e95a-4124-84a1-7f2e5e384e5a.png

    Data Delivery File Formats


    Fastq Data Files

    Sequencing Yield Display


    Plant leaf:

    756cad2f22bd5960e5c24a69f33875db_637cca1146b3573d1dbea7fc398cfac869c4ce35719d5.png

    Mouse embryo:

    51157adb1e1736e7174876626aeaccba_26813aa5a82e46eac252f403b8bd858469c4ce4848672.jpg

    Sample Requirements


    Technical Type Sample Type Sample Collection Transportation
    Single-cell RNA Sequencing Animal

    Quantity Requirements:
    Parenchymal Organ Tissues (lung, liver, gland, etc.)≥ 200 mg
    Adipose Tissue, Blood Vessels, Heart Valves, Muscle, etc.≥ 500 mg
    Criteria: remove locally necrotic or damaged areas caused by electric scalpel, laser ablation, or spontaneous tissue necrosis, as well as non-target tissue components.

    Snap-freeze in liquid nitrogen for 20–30 minutes, then store at -80°C or ship immediately.

    Pack the sorted samples and embed them in dry ice and send it out as soon as possible.Ensure sufficient dry ice is used.

    Plants Quantity Requirements: 1–2 g
    Criteria: Prioritize tender, well-growing, and representative plants.
    Snap-freeze in liquid nitrogen for 30 minutes, then store at -80°C or ship immediately.

    Publications


    Year Journal

    Impact Factor

    Title
    2025 Nature Neuroscience 20 GABA-dependent microglial elimination of inhibitory synapses underlies neuronal hyperexcitability in epilepsy
    2025 Nature Cancer 28.5 Skeletal muscle endothelial dysfunction through the activin A–PGC1α axis drives progression of cancer cachexia
    2025 Nature Immunology 27.6 Bat organoids reveal antiviral responses at epithelial surfaces
    2025 Immunity 26.3 Maternal natural killer cells drive neuroimmune disorders in offspring through aberrant secretion of extracellular granzyme B
    2025 Cell 42.5 Post-gastrulation amnioids as a stem cell-derived model of human extra-embryonic development

    What's the best choice?


     

    Long-read Single-Cell Transcriptome Sequencing

    Learn more>>>

    Single-cell transcriptome sequencing

    Sequencing Technology Nanopore PromethION Illumina
    Reads Length Reads N50:~1kb 150bp
    Delivery File Format Fastq Fastq
    alyze Content

    1、Gene expression,cell clustering,cell annotation

    2、Identification of isoform diversity

    3、Isoform quantification

    4、Differential transcript analysis for cell clustering

    5、Comparison of gene clustering results with isoform clustering results

    6、Identification of cell subpopulations through differential alternative splicing

    7、Isoform switching between cell populations

    8、Identification of fusion genes in cell clusters

    9、Identification of IncRNAs in cell clusters

    1、Gene expression, cell clustering,cell annotation

    Contact Us

    If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.

    Contact Us
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    Contact Us

    E-mail:service@sailgene.com

    WhatsApp:1-(617)-223-7544

    Tel:16172237544

    Email:service@sailgene.com

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