Sailgene Technology
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Sailgene Technology
Introduction
ATAC-seq (Assay for Transposase Accessible Chromatin with High-Throughput Sequencing) is a high-throughput sequencing technology used to study chromatin accessibility. This method leverages the Tn5 transposase, which recognizes and binds to open chromatin regions. It simultaneously cuts the DNA at these regions and inserts sequencing adapters at both ends of the cut DNA fragments. High-throughput sequencing is then performed to identify open chromatin regions across the genome. This technique is commonly used to investigate how chromatin structure influences gene transcriptional regulation.
Applications
Mapping Open Chromatin Regions
Predicting Transcription Factor Binding Sites and Regulatory Elements
Highlights
High Sensitivity
Versatility
High-Resolution
Efficiency
Library Construction Workflow
Analysis Workflow
Data Delivery File Formats
Fastq Data Files
Data Delivery Standard
Sample Requirements
|
Sample Type Platform |
Plant tissue sample |
Animal tissue sample |
Number of cells cultured |
Blood |
fungi |
|
ATAC-seq（Illumina）
|
≥500 mg |
≥500 mg |
≥2*105 |
≥1 mL |
≥500 mg |
Publications
The gene regulatory landscape driving mouse gonadal supporting cell differentiation （Science Advances, IF=12.5）
Article link：
https://www.science.org/doi/10.1126/sciadv.adv1885
Abstract：Gonadal sex determination relies on tipping a delicate balance involving the activation and repression of several transcription factors and signaling pathways. This is likely mediated by numerous noncoding regulatory elements that shape sex-specific transcriptomic programs. To explore the dynamics of these in detail, we performed paired time series of transcriptomic and chromatin accessibility assays on pre-granulosa and Sertoli cells throughout their development in the embryo, making use of new and existing mouse reporter lines. Regulatory elements were associated with their putative target genes by linkage analysis, and this was complemented and verified experimentally using promoter capture Hi-C. We identified the transcription factor motifs enriched in these regulatory elements along with their occupancy, pinpointing LHX9/EMX2 as potentially critical regulators of ovarian development. Variations in the DNA sequence of these regulatory elements are likely to be responsible for many of the unexplained cases of individuals with differences of sex development.
What's the best choice?
|
Metric |
ATAC-seq |
DNase-seq |
MNase-seq |
|
Target |
Assessing chromatin accessibility regions |
Identifying DNase I cleavage sites in open chromatin |
Assessing nucleosome occupancy in chromatin |
|
Experimental Principle |
Using transposase to insert barcodes into accessible chromatin regions |
Using DNase I to cleave open chromatin, sequencing cleavage sites |
Using micro amounts of MNase to digest chromatin and sequencing nucleosome cleavage sites |
|
Resolution |
High (smaller chromatin fragments) |
Medium (cleavage fragments typically 150-200 bp) |
Low (typically 150-200 bp, dependent on nucleosome spacing) |
|
Applications |
Identifying accessible chromatin regions, regulatory element studies |
Identifying functional DNA regions, regulatory regions of genes |
Nucleosome positioning, chromatin structure studies, transcription regulation studies |
Contact Us
If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.
Contact Us
Add: 82 Wendell Avenue, Pittsfield, Massachusetts, USA
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