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    Classification:

    Telomere-to-Telomere (T2T) Genome


    Introduction


    The Telomere-to-Telomere (T2T) genome represents a complete and gapless assembly of anorganism's genome, spanning from one telomere to the other. Unlike conventional assembliesthe T2T genome leverages advanced long-read sequencing technologies-PacBio HiFi and Nanopore ultra-long reads-in combination with Hi-C scaffolding to produce highly contiguousaccurate, and comprehensive genomic assemblies. As a gold standard in genome assemblythe T2T genome provides a powerful foundation for investigating genome architecture,evolutionary biology, functional genomics, and unlocking new frontiers in genomic research.

    Applications


    Segmental duplication and variation research

    Research on genes located near centromere

    Functional gene identification & genetic variation analysis

    Epigenetic research

    Centromere diversity research

    Highlights


    Independently developed Nanopore ultra-long DNA extraction kits and successfully sequenced over 200 species using Nanopore ultra-long read technology.

    Developed and patented a proprietary telomere-to-telomere (T2T) genome assembly method.

    Published 20+ high-impact T2T genome studies in leading joumals over the past two years.

    Workflow


    Sample Preparation

    Sample Preparation

    Refer to: Sample Submission Guidelines

    Extraction

    Extraction

    Refer to Ultra-long DNA Extraction Protocol / DNA Extraction Protocol

    Sample QC

    Extraction
    Library Preparation

    Library Preparation

    Refer to Ultra-long DNA Library Construction Workflow / PacBio Revio DNA Library Construction Workflow

    Library QC

    Library Preparation
    Sequencing

    Sequencing

    Platform: -Short-read Sequencing: Illumina/DNBSEQ; -Long-read Sequencing: Nanopore PromethION / PacBio Revio

    Data QC

    Sequencing
    Bioinformatics Analysis

    Bioinformatics Analysis

    Pan-genome Demo Report

    Click >>

    Analysis Workflow


    Telomere-to-Telomere (T2T) Genome.png

    Publications


    Year

    Journal

    Impact Factor

    Title

    2025

    nature genetics

    31.8

    A telomere-to-telomere genome assembly coupled with multi-omic data provides  insights into the evolution of hexaploid bread wheat

    2025

    Molecular Plant

    24.1

    Two complete telomere-to-telomere Medicago genomes reveal the landscape and evolution of centromeres

    2025

    Nature Communications

    16.6

    Comparative genomics provides insights into the biogeographic and biochemical diversity of meliaceous species

    2025

    Plant Communications

    11.6

    A telomere-to-telomere reference genome assembly of tomato cultivar Heinz 1706

    2025

    Plant Biotechnology Journal

    12.1

    A telomere-to-telomere gap-free assembly integrating multi-omics uncovers the genetic mechanism of fruit quality and important agronomic trait associations in pomegranate

    Contact Us

    If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.

    Contact Us
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    Contact Us

    E-mail:service@sailgene.com

    WhatsApp:1-(617)-223-7544

    Tel:16172237544

    Email:service@sailgene.com

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