Sailgene Technology
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Sailgene Technology
Introduction
Nanopore platforms enable single-molecule sequencing by directly detecting changes in electrical current as DNA strands pass through nanopores. This technology supports read lengths of hundreds of kilobases (kb). When combined with Sailgene’s proprietary ultra-long DNA extraction kit, read lengths with an N50 exceeding 150 kb can be achieved.
Applications
Whole genome sequencing
T2T genome assembly
Identification of genetic structure variations
Improved continuity of assembly
Human genetic diseases research
Highlights
Sequencing produces ultra-long reads directly, eliminating the need for fragment screening
Sequencing reads N50 up to 100-200 kb
Samples from various eukaryotic organisms are accepted
Addressing the sequencing challenges of complex regions
Workflow
Bioinformatics Ananlysis
Demo Report
Library Construction Workflow
Data Delivery File Formats
Fastq and POD5 Data Files
Data Delivery Standard
|
|
Sample Types |
Data yield per cell (Gb) (N50 > 150K) |
|
Conventional species |
Plants |
rice, maize, wheat, soybean, peanut, etc.: >15G Conventional forest trees: >10G |
|
Animals |
Mammals, including humans, primates, pigs, cattle, etc.: fresh blood >15G |
Sequencing Yield Display
|
Kingdom |
Family/Category |
Data yield per cell (Gb)(N50>150K) |
Q |
|
Plants |
Ericaceae |
20 |
24 |
|
Salicaceae |
17 |
23 |
|
|
Rosaceae |
22 |
24 |
|
|
Brassicaceae |
20 |
24 |
|
|
Moraceae |
25 |
24 |
|
|
Fabaceae |
15 |
24 |
|
|
Solanaceae |
16 |
24 |
|
|
Poaceae |
16 |
24 |
|
|
Amaranthaceae |
16 |
24 |
|
|
Animals |
Mammal |
20 |
24 |
|
Freshwater fish |
15 |
24 |
|
|
Inset |
10 |
24 |
Sample Requirements
1.Plant samples are recommended to send newly grown young leaves.
2.Animal samples are recommended to give preference to fresh blood, followed by liver and other tissues.
3.Unconventional samples do not guarantee sequencing yield.
Nanopore sequencing unconventional samples are as follows:
1) plants with a lot of secondary metabolites;
2) oceans and aquatic products (animals and plants, algae, etc.);
3) amphibians, birds, etc.
Publications
| Year | Journal | Impact Factor | Title |
| 2025 | nature genetics | 31.8 | A telomere-to-telomere genome assembly coupled with multi-omic data provides insights into the evolution of hexaploid bread wheat |
| 2025 | Molecular Plant | 24.1 | Two complete telomere-to-telomere Medicago genomes reveal the landscape and evolution of centromeres |
| 2025 | Nature Communications | 16.6 |
Comparative genomics provides insights into the biogeographic and biochemical diversity of meliaceous species |
| 2025 | Plant Communications | 11.6 | A telomere-to-telomere reference genome assembly of tomato cultivar Heinz 1706 |
| 2025 | Plant Biotechnology Journal | 12.1 |
A telomere-to-telomere gap-free assembly integrating multi-omics uncovers the genetic mechanism of fruit quality and important agronomic trait associations in pomegranate |
Keyword:
Nanopore Ultra-long Sequencing (PromethION platform)
Contact Us
If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.
Contact Us
Add: 82 Wendell Avenue, Pittsfield, Massachusetts, USA
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