Full-Length Transcriptome Sequencing & Nanopore‑based Isoform Analysis

Full-Length Transcriptome Sequencing, which bypasses fragmentation, directly generates high-quality full-length sequences spanning 5' to 3' of reverse transcribed cDNA using the ONT sequencing platform. This approach not only enables precise analysis of alternative splicing events and fusion genes but also facilitates the identification and quantification of novel isoforms, thus ensuring accurate measurements of transcript expression levels.

Applications

Developmental Biology Research

Oncology Research

Immunoinfection Research

Environmental Toxicology Research

Highlights

High sequencing quality, fast turnaround time, and cost-effective pricing.

Enables the discovery of novel transcripts and accurate quantification of transcript levels.

Quantifies alternative splicing events.

Long nanopore reads can span full-length transcripts simplifying their identification

Library Construction Workflow

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Analysis Workflow

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Data Delivery File Formats

FASTQ Data Files

Sample Requirements

Sample Types	Amount	RNA Integrity	Purity
Total RNA	≥0.8 μg	The RNA sample is clear and transparent, with no insoluble matter. The 28S and 18S main bands are clear, with 28S/18S ratio ≥ 1, and the insect 18S band is clear without any trailing.	A260/280=1.9-2.1; A260/230≥1.0

Tissue Type	Full-length Transcriptome Sequencing
Dry Weight of Fresh Animal Tissue	≥300 mg
Dry Weight of Fresh Plant Tissue	≥500 mg
Freshly Cultured Cells	≥1-5×10⁶
Freshly Collected Whole Blood	2.5-5 ml
Microbial Biomass	≥5×10⁶or ≥0.2 g

Publications

Year	Journal	^{Impact Factor}	Title
2025	Cell	45.5	De novo assembly of nuclear stress bodies rearranges and enhances NFIL3 to restrain acute inflammatory responses
2025	Advanced Science	14.3	Targeting PTBP3-Mediated Alternative Splicing of COX11 Induces Cuproptosis for Inhibiting Gastric Cancer Peritoneal Metastasis
2025	Molecular Cell	14.5	RIOK3 mediates the degradation of 40S ribosomes
2024	Horticulture Research	7.6	Full-length transcriptome sequencing of pepper fruit during development and construction of a transcript variation database

What's the best choice?

	Full-length transcript sequencing	Tail Iso-seq	Direct RNA-seq Learn more>>>	Direct cDNA-seq	Full-Length lncRNA Sequencing Learn more>>>	Long non-coding RNAs Sequencing	PacBio iso-seq	RNA-seq
Gene quantification	+++	+++	+++	+++	+++	+++	+	+++
Gene difference analysis	+++	+++	+++	+++	+++	+++	+	+++
Isoform quantification	+++	+++	+++	+++	+++	+	+	+
Isoform difference analysis	+++	+++	+++	+++	+++	+	+	+
Alternative splicing	+++	+++	+++	+++	+++	+	+++	+
fusion gene	+++	+++	+++	+++	+++	+	+++	+
novel transcript prediction	+++	+++	+++	+++	+++	+	+++	+
Allele analysis	+++	+++	+++	+++	+++	NO	+++	+
Alternative polyadenylation	+++	+++	+++	+++	+++	+	+++	+
Poly(A) length	NO	+++	+++	NO	NO	NO	NO	NO
LncRNA analysis	+	+	+	+	+++	+++	NO	+
Modification analysis (m6A, m5C, Ψ, I)	NO	NO	+++	NO	NO	NO	NO	NO
Positive and negative chain analysis	+++	+++	+++	+++	+++	+++	+	NO
Library Construction	Reverse transcription and PCR amplification	Reverse transcription and PCR amplification	Without reverse transcription and PCR amplification	Only reverse transcription	Reverse transcription and PCR amplification	Reverse transcription and PCR amplification	Reverse transcription and PCR amplification	Reverse transcription and PCR amplification
Sequencing platform	Nanopore PromethION	Nanopore PromethION	Nanopore PromethION	Nanopore PromethION	Nanopore PromethION	Illumina	^{PacBio Sequel II}	Illumina

Keyword:

Full-length Transcriptome Sequencing

Contact Us

If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.

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Tel：1-(617)-223-7544

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Add: 82 Wendell Avenue, Pittsfield, Massachusetts, USA