Sailgene Technology
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Sailgene Technology
Introduction
Direct RNA Sequencing: Without reverse transcription or amplification, it enables direct reading of full-length poly(A)-tailed RNA molecules (rather than cDNA). This technology can detect RNA modification sites (e.g., m6A, m5C, pseudouridine ψ, inosine, and 2'-O-Me) on individual RNA molecules, enabling precise analysis of alternative splicing, fusion genes, and the identification of novel isoforms. Additionally, it provides relatively accurate estimation of poly(A) tail length, thereby preserving the native RNA characteristics.
Applications
Developmental Biology Research
Oncology Research
Immunoinfection Research
Environmental Toxicology Research
Highlights
Detect RNA modifications (m5C, m6A, ψ, inosine, 2'-O-Me, etc.) at single-nucleotide resolution.
Accurately estimate poly(A) tail length.
Avoid the introduction of false-positive transcripts via direct RNA sequencing without reverse transcription or amplification.
Library Construction Workflow
Analysis Workflow
Data Delivery File Formats
FASTQ and POD5 Data Files
Sequencing Yield Display
| Species | TotalBase | TotalReads | N50 | meanQ |
| Human | 24,186,756,830 | 21,156,455 | 1,685 | 21.49 |
| Human | 26,236,107,976 | 20,699,814 | 1,781 | 21.79 |
| Plant | 16,221,740,860 | 18,232,141 | 958 | 21.59 |
| Animal | 24,079,670,614 | 24,346,060 | 1,213 | 21.56 |
| Animal | 25,781,032,891 | 24,731,182 | 1,404 | 22 |
| Animal | 20,875,860,663 | 17,684,923 | 1,681 | 22 |
| Animal | 19,551,477,538 | 20,416,360 | 1,296 | 21.65 |
| Animal | 20,325,290,079 | 18,077,586 | 1,589 | 21.71 |
| Cell | 22,865,483,808 | 17,417,836 | 1,938 | 20.43 |
| Mammal | 20,875,860,663 | 17,684,923 | 1,681 | 22 |
| Mammal | 20,325,290,079 | 18,077,586 | 1,589 | 21.71 |
| Mammal | 22,710,639,651 | 23,941,576 | 1,296 | 21.65 |
| Plant | 19,398,123,548 | 21,691,091 | 1,209 | 21.84 |
| Plant | 18,097,091,216 | 20,688,982 | 1,161 | 21.34 |
Sample Requirements
|
Sample Types |
Amount | RNA Integrity | Purity |
| Total RNA | Data>10Gb: ≥15 μg; Data<10Gb: ≥6 μg; |
The RNA sample is clear and transparent, with no insoluble matter. The 28S and 18S main bands are clear, with 28S/18S ratio ≥ 1, and the insect 18S band is clear without any trailing. | A260/280=1.9-2.1; A260/230≥1.0 |
|
Tissue Type |
Direct RNA Sequencing |
|
Dry Weight of Fresh Animal Tissue |
≥1 g |
|
Dry Weight of Fresh Plant Tissue |
≥2 g |
|
Freshly Cultured Cells |
≥1-5×10⁷ |
|
Freshly Collected Whole Blood |
10-15 ml |
|
Microbial Biomass |
≥5×10⁷ or ≥0.2 g |
Publications
| Year | Journal |
Impact Factor |
Title |
| 2025 |
Nature Communications |
15.7 |
A mutually antagonistic mechanism mediated by RNA m6A modification in plant-virus interactions |
| 2025 | Science Bulletin | 21.1 |
Epi-transcriptome analysis reveals the lack of N6-methyladenosine modification in two RNA viruses infecting watermelon |
| 2025 | Genome Biology | 10.1 |
Widespread impact of transposable elements on the evolution of post-transcriptional regulation in the cotton genus Gossypium |
| 2025 | Molecular Cell | 14.5 |
Single-molecule m6A detection empowered by endogenous labeling unveils complexities across RNA isoforms |
| 2025 | Developmental Cell | 10.7 |
Nanopore RNA direct sequencing identifies that m6A modification is essential for sorbitol-controlled resistance to Alternaria alternata in apple |
| 2024 |
Signal Transduction and Targeted Therapy |
40.8 | Characterization of ACTN4 as a novel antiviral target against SARS-CoV-2 |
What's the best choice?
|
Full-length transcript sequencing |
Tail Iso-seq | Direct RNA-seq | Direct cDNA-seq |
Full-Length lncRNA Sequencing |
Long non-coding RNAs Sequencing |
PacBio iso-seq |
RNA-seq |
|
|
Gene quantification |
+++ | +++ | +++ | +++ | +++ | +++ | + | +++ |
|
Gene difference analysis |
+++ | +++ | +++ | +++ | +++ | +++ | + | +++ |
|
Isoform quantification |
+++ | +++ | +++ | +++ | +++ | + | + | + |
|
Isoform difference analysis |
+++ | +++ | +++ | +++ | +++ | + | + | + |
|
Alternative splicing |
+++ | +++ | +++ | +++ | +++ | + | +++ | + |
|
fusion gene |
+++ | +++ | +++ | +++ | +++ | + | +++ | + |
|
novel transcript prediction |
+++ | +++ | +++ | +++ | +++ | + | +++ | + |
|
Allele analysis |
+++ | +++ | +++ | +++ | +++ | NO | +++ | + |
|
Alternative polyadenylation |
+++ | +++ | +++ | +++ | +++ | + | +++ | + |
|
Poly(A) length |
NO | +++ | +++ | NO | NO | NO | NO | NO |
|
LncRNA analysis |
+ | + | + | + | +++ | +++ | NO | + |
|
Modification analysis (m6A, m5C, Ψ, I) |
NO | NO | +++ | NO | NO | NO | NO | NO |
|
Positive and negative chain analysis |
+++ | +++ | +++ | +++ | +++ | +++ | + | NO |
|
Library Construction |
Reverse transcription and PCR amplification | Reverse transcription and PCR amplification | Without reverse transcription and PCR amplification | Only reverse transcription | Reverse transcription and PCR amplification | Reverse transcription and PCR amplification | Reverse transcription and PCR amplification | Reverse transcription and PCR amplification |
|
Sequencing platform |
Nanopore PromethION | Nanopore PromethION | Nanopore PromethION | Nanopore PromethION | Nanopore PromethION | Illumina | PacBio Revio | Illumina |
Contact Us
If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.
Contact Us
Add: 82 Wendell Avenue, Pittsfield, Massachusetts, USA
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